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Effect of 0.5 mM SCFA ± LPS stimulation on L6 myotube inflammatory mediator mRNA expression (A–C) and LPS-stimulated phosphorylated transcription factor intracellular protein levels (D,E). Values are means ± SEM (n = 8/group). Data were analyzed by two-way ANOVA (A–C), or one-way ANOVA (D,E) followed by Tukey’s multiple comparison test. Bars not sharing a lower-case letter differ (p < 0.05). SCFA treatment without LPS (black bars) and SCFA with LPS (grey bars).
