Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 16;23(14):7856. doi: 10.3390/ijms23147856

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunohistochemistry of the masseter muscle in the control group. (a) Anti-BAF8 (red) was used for the staining of type 1 muscle fibers, and almost all specimens were negative. (b) Anti-SC71 (orange) staining was performed to detect type 2A fibers, and a slightly positive area was observed. (c) Anti-BFF3 (green) staining suggested that most of the masseter muscle fibers were composed of type 2B fibers. Nuclear counterstaining was performed using DAPI (blue). (d) Anti-BAF8-, anti-AC71-, and anti-BFF3-positive areas were analyzed with four specimens. The average percentage area of type 1, type 2A, type 2B and the estimated type 2X fibers were 2.92 ± 2%, 4.40 ± 3.62%, 41.52 ± 6.30% and 51.16 ± 6.77%, respectively. This indicated that the original masseter muscle was composed mainly of type 2 fibers, especially type 2B fast-twitch glycolytic muscle fibers (n = 4 each, * p < 0.01, one-way ANOVA).