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. 2022 Jun 27;20(7):418. doi: 10.3390/md20070418

Figure 7.

Figure 7

Mandelalide L induces time and concentration-dependent phosphorylation of AMPK and ACC. (A) Immunoblot analysis of U87-MG glioblastoma cells treated with mandelalide A for 30 min. Whole cell lysates were probed with antibodies for phospho-AMPK, phospho-ACC, total AMPK, total ACC and GAPDH as indicated. Blots are representative of a single experiment that was repeated three times. (B) Histograms show quantification of immunoblot data shown in A from three independent experiments; phospho-AMPK/total AMPK and phospho-ACC/total ACC, normalized to loading control (C) Immunoblot analysis of U87-MG treated with 1 μM of mandelalide L for 30 min, 1 h or 4 h and probed with antibodies shown in A. (D) Histograms show the quantification of immunoblot data shown in C from three independent experiments; phospho-AMPK/total AMPK and phospho-ACC/total ACC, normalized to loading control. Statistical significance of change relative to vehicle (0.1% DMSO) is indicated in B and D as * p < 0.05, ** p < 0.01, *** p < 0.001.