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Effect of hydrogenase inhibitors or hydrogenase contents on Tc(VII) reduction in D. fructosovorans. Cells were incubated at 23°C and pH 5.5 with hydrogen supplied as an electron donor. Open circles, wild-type strain (control); closed circles, wild-type strain after preincubation with air; closed squares, wild-type strain after preincubation with 0.5 mM CuCl2; closed triangles, mutant MR400 with the nickel-iron hydrogenase genes deleted; open triangles, complemented mutant MR400.
