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PFGE of DNA fragments from freshly prepared parent cells and the 1- and 2-year-old isolates. The chromosomal DNA from each was isolated, digested with SmaI, and subjected to PFGE. (a) Parent cells (lane 1), the 1-year-old LCT isolate (lane 2), the 1-year-old TCT isolate (lane 3), and the 1-year-old OCT isolate (lane 4) are shown. (b) Parent cells (lane 1) and 2-year-old isolates (lanes 2 to 5) are shown.
