Fig. 5.

Mutated SNF4 versions no longer suppress KINβ2 inhibition of KIN10 activity. DIN6 promoter activity in Arabidopsis thaliana leaf mesophyll protoplasts upon transient expression of Arabidopsis KIN10, KINβ2 and SNF4 or SNF4‐V449M or SNF4‐A418T 6 h after transfection. Values are averages with standard deviations (n = 4). ANOVA Tukey’s multiple comparisons test was applied, and letters represent the statistical differences (P < 0.001). Protein expression was assessed by immunoblot analysis with anti‐HA and anti‐FLAG antibodies. RBSC served as a loading control.