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. 2022 Jul 12;23(14):7673. doi: 10.3390/ijms23147673

Figure 3.

Figure 3

Effect of AdoMet alone and in combination with miR-34a, miR-34c, and miR-449a mimics on the levels of migration- and EMT-related proteins in CRC cells. Cells were transfected with 100 nM miR-34a, miR-34c, and miR-449a in the presence or not (control) of 500 μM AdoMet for 48 h. The expression levels of MMP2, MMP9, E-cadherin, N-cadherin, vimentin, and β-catenin, were detected by Western blot using the total cell lysate of HCT-116 (A) and Caco-2 (B) cells. The densitometric analysis was reported as the percentage of protein expression of untreated control (100%). For the equal loading of proteins in the lanes, β-actin was used as a standard. The images are representative of three immunoblotting analyses obtained from at least three independent experiments. The means were compared using analysis of variance (ANOVA) plus Bonferroni’s t-test. * p < 0.05, ** p < 0.01 versus untreated cells (Control). Uncropped images of Western blots are reported in Figures S1 and S2.