FIGURE 7.

SftA overproduction in ∆ezrA does not affect Z‐ring formation. Fluorescence microscopy images of ∆ezrA cells overexpressing SftA from an ectopic locus by induction with 1% xylose for 1 h. Localization of the early cell division proteins YFP‐FtsA and YFP‐SepF is shown in the upper and middle panel, respectively, and the localization of the late cell division protein YFP‐PbpB is shown in the lower panel. Some elongated cells that were blocked in cell division still formed YFP‐PbpB bands (arrow). Reporter fusions were induced with 0.2 mM IPTG, and membranes were stained with FM5‐95. Scale bars are 2 μm. Strains used: TNVS710, TNVS712, and TNVS713, respectively