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. 2022 Apr 30;117(5):1263–1274. doi: 10.1111/mmi.14906

FIGURE 8.

FIGURE 8

DNA bisection and sensitivity to ciprofloxacin. (a) Chromosome bisection in B. subtilis ∆sftA and ∆sepF mutants. A microscopic example is presented in the left panel. The table indicates the percentage of bisection based on approximately 500–1000 cells from independent biological triplicates. Membrane and DNA were stained with FM5‐95 and DAPI, respectively. The scale bar is 2 μm. Strains used: wild type (wt), TNVS083, TNVS159, and TNVS152, respectively. (b) Spot dilution assay showing the sensitivity of different B. subtilis mutants for the topoisomerase IV inhibitor ciprofloxacin. Strains used: wild type (wt), TNVS083, TNVS089, TNVS152, TNVS159, TNVS371, TNVS373, and TNVS431, respectively