Figure 1.

Undetectable/unresolvable minor donor analysis scheme. Standard “bulk” sampling results in a DNA profile that appears likely single source for individual A where individual B’s genotype cannot be distinguished (left side). The minor donor (B) is undetectable/barely detectable (left side). Simplified micromanipulation subsampling (DSCS) of the same mixture using a 5-cell subsampling size results in single source profiles of individual A and 1:4 mini-mixture samples of individuals A and B (right side, A is major donor and B is the minor donor)). These mini-mixture subsamples are then analyzed with PG validated for one or few cells.