TABLE 1.
Results of RT-PCR inhibitor elimination assays performed with raw sewage and sewage-polluted creek water samples
| Sampling
|
Result of snPCR ofa:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Site | Date (mo/day/yr) | Autoclaving | In natura water
|
Water purified by:
|
||||||
| Vertrel XF
|
ZP60S filtration
|
ZP60S filtration + Vertrel XF
|
||||||||
| NS | S | NS | S | NS | S | NS | S | |||
| Creek | 1/27/98 | No | − | + | + | + | − | + | − | + |
| Yes | + | + | − | + | − | + | − | + | ||
| 1/28/98 | No | − | + | − | + | − | + | − | + | |
| Yes | + | + | + | + | − | + | − | + | ||
| 2/02/98 | No | + | + | − | + | − | + | − | + | |
| Yes | − | + | + | + | − | + | − | + | ||
| Sewage | 6/03/98 | No | + | + | − | + | − | + | − | + |
| Yes | − | + | − | + | − | + | − | + | ||
| 6/17/98 | No | − | − | − | − | − | + | − | + | |
| Yes | + | + | − | + | − | + | − | + | ||
| 6/24/98 | No | + | + | + | + | − | + | − | + | |
| Yes | − | + | − | + | − | + | − | + | ||
a
Rotavirus gene amplification. +, detected (absence of inhibitors); −, none detected. NS, samples were not seeded with rotavirus SA-11; S, samples were seeded with rotavirus SA-11.