TABLE 1.
Analysis of variance. Multivariate (MANOVA) and univariate (ANOVA) analysis of variance, testing the effect of generation (G), genotype (Gen), treatment (T) and their interaction terms on five fitness‐linked life history traits. The chemical tested are: PFOS (70 ng L−1), diclofenac (2 mg L−1), trimethoprim (2 mg L−1), atrazine (0.2 mg L−1) and arsenic (1,000 µg L−1). Size at maturity (mm), age at maturity (days), fecundity (number of offspring across two broods), interval between broods (time elapsed between broods) and mortality were measured. Each genotype is run in five clonal replicates. Significant p‐values are in bold
| MANOVA | ANOVA | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Size at maturity (mm) | Age at maturity (days) | Fecundity | Interval between broods | Mortality | |||||||||||
| df | F | p‐Value | df | Chi‐sq | p‐Value | Chi‐sq | p‐Value | Chi‐sq | p‐Value | Chi‐sq | p‐Value | Chi‐sq | p‐Value | ||
| PFOS | |||||||||||||||
| Generation (G) | 2 | 9.04 | 3.2e‐09*** | Generation (G) | 2 | 41.68 | 8.9e‐10*** | 41.94 | 7.8e‐10*** | 62.40 | 2.8e‐14*** | 8.10 | .02* | 6.48 | .010891* |
| Genotype (Gen) | 3 | 4.31 | 7.4e‐06*** | Genotype (Gen) | 3 | 5.14 | .16 | 2.61 | .46 | 29.19 | 2.0e‐06*** | 0.34 | .95 | 16.16 | .001052** |
| Treatment (T) | 1 | 2.69 | .04* | Treatment (T) | 1 | 19.74 | 8.8e‐06*** | 0.49 | .48 | 4.09 | .04304* | 0.03 | .87 | 5.18 | .022829* |
| G × Gen | 4 | 1.59 | .07 | G × Gen | 4 | 18.74 | .0009*** | 9.17 | .06 | 33.20 | 1.1e‐06*** | 1.29 | .86 | 5.69 | .13 |
| G × T | 2 | 3.07 | .004** | G × T | 2 | 13.29 | .001** | 23.12 | 9.5e‐06*** | 7.44 | .02* | 1.72 | .42 | 1.87 | .17 |
| Gen × T | 3 | 1.94 | .03* | Gen × T | 3 | 22.85 | 4.3e‐05*** | 0.54 | .91 | 1.28 | .73 | 1.92 | .59 | 8.98 | .029603* |
| G × Gen × T | 4 | 1.19 | .27 | G × Gen × T | 4 | 7.03 | .13 | 1.52 | .82 | 4.45 | .35 | 1.29 | .86 | 8 | .038836* |
| DICLOFENAC | |||||||||||||||
| Generation (G) | 2 | 14.77 | 2.1e‐15*** | Generation (G) | 2 | 73.63 | <2.2e‐16*** | 110.90 | <2.2e‐16*** | 127.47 | <2.2e‐16*** | 21.35 | 2.3e‐05*** | 7.85 | .0197461* |
| Genotype (Gen) | 3 | 2.67 | .002** | Genotype (Gen) | 3 | 13.25 | .004** | 6.34 | .10 | 29.37 | 1.9e‐06*** | 0.50 | .92 | 7.76 | .05 |
| Treatment (T) | 1 | 16.24 | 2.4e‐09*** | Treatment (T) | 1 | 44.52 | 2.5e‐11*** | 39.40 | 3.4e‐10*** | 16.32 | 5.3e‐05*** | 0.12 | .73 | 13.45 | .0002447*** |
| G × Gen | 5 | 2.37 | .001** | G × Gen | 5 | 26.05 | 8.7e‐05*** | 17.25 | .004** | 56.96 | 5.2e‐11*** | 3.43 | .63 | 14.84 | .0110460* |
| G × T | 2 | 10.81 | 1.0e‐11*** | G × T | 2 | 31.09 | 1.8e‐07*** | 83.62 | <2.2e‐16*** | 21.13 | 2.6e‐05*** | 5.76 | .06 | 21.33 | 2.331e‐05*** |
| Gen × T | 3 | 2.59 | .003** | Gen × T | 3 | 13.52 | .004** | 5.42 | .14 | 2.00 | .57 | 2.82 | .42 | 1.97 | .58 |
| G × Gen × T | 5 | 3.51 | 1.370e‐06*** | G × Gen × T | 5 | 25.37 | .0001*** | 17.25 | 3.5e‐07*** | 8.84 | .12 | 5.35 | .37 | 0 | 1 |
| TRIMETHOPRIM | |||||||||||||||
| Generation (G) | 2 | 16.77 | <2.2e‐16*** | Generation (G) | 2 | 16.65 | .0002*** | 87.14 | <2.2e‐16*** | 92.88 | <2.2e‐16*** | 21.85 | 1.8e‐05*** | 0.10 | .76 |
| Genotype (Gen) | 3 | 4.35 | .55 | Genotype (Gen) | 3 | 18.29 | .0004*** | 18.29 | .0003827*** | 39.90 | 1.1e‐08*** | 1.35 | .72 | 7.01 | .14 |
| Treatment (T) | 1 | 1.45 | .22 | Treatment (T) | 1 | 0.25 | .62 | 0.34 | .56 | 0.07 | .79 | 4.20 | .04* | 0.94 | .33 |
| G × Gen | 6 | 2.31 | .0006*** | G × Gen | 6 | 21.26 | .002** | 17.84 | .006637** | 28.44 | 7.76e‐05*** | 4.19 | .65 | 2.76 | .43 |
| G × T | 2 | 2.30 | .023* | G × T | 2 | 0.90 | .64 | 7.55 | .022895* | 4.17 | .12 | 1.61 | .45 | 0.04 | .85 |
| Gen × T | 3 | 3.39 | .0001*** | Gen × T | 3 | 29.47 | 1.8e‐06*** | 3.78 | .29 | 16.34 | .0001*** | 2.43 | .49 | 2.63 | .45 |
| G × Gen × T | 6 | 1.75 | .02* | G × Gen × T | 6 | 16.89 | .01** | 9.25 | .16 | 12.92 | .04* | 7.95 | .24 | 9.23 | .02639* |
| ATRAZINE | |||||||||||||||
| Generation (G) | 2 | 7.07 | .84 | Generation (G) | 2 | 44.74 | 1.9e‐10*** | 39.83 | 2.2e‐09*** | 35.42 | 2e‐08*** | 3.88 | .14 | 0.01 | .90 |
| Genotype (Gen) | 3 | 1.50 | .39 | Genotype (Gen) | 3 | 23.06 | 3.9e‐05*** | 3.98 | .26 | 21.28 | 9.2e‐05*** | 0.82 | .84 | 12.87 | .004920** |
| Treatment (T) | 1 | 15.46 | 1.4e‐07*** | Treatment (T) | 1 | 89.50 | <2.2e‐16*** | 0.52 | .47 | 33.65 | 6.6e‐09*** | 0.18 | .68 | 20.53 | 5.881e‐06*** |
| G × Gen | 2 | 2.49 | .02* | G × Gen | 2 | 10.06 | .006** | 2.35 | .31 | 8.69 | .01294* | 0.12 | .94 | 4.38 | .11 |
| G × T | 2 | 2.19 | .04* | G × T | 2 | 6.42 | .04* | 3.55 | .17 | 1.62 | .44 | 0.67 | .71 | 0.14 | .71 |
| Gen × T | 3 | 2.24 | .55 | Gen × T | 3 | 4.18 | .24 | 3.84 | .28 | 1.73 | .63 | 1.54 | .67 | 4.96 | .18 |
| G × Gen × T | 1 | 2.76 | .04* | G × Gen × T | 2 | 7.12 | .03* | 2.84 | .03* | 6.52 | .04* | 0.00 | .99 | 10.2274 | .006014** |
| ARSENIC | |||||||||||||||
| Generation (G) | 2 | 14.17 | 4.233e‐15*** | Generation (G) | 2 | 83.76 | <2.2e‐16*** | 39.14 | 3.165e‐09*** | 0.35 | .84 | 8.26 | .0161066* | 2.53 | .28 |
| Genotype (Gen) | 3 | 4.15 | 7.332e‐06*** | Genotype (Gen) | 3 | 17.58 | .0005378*** | 134.18 | <2.2e‐16*** | 38.44 | 2.284e‐08*** | 6.25 | .10 | 1.01 | .80 |
| Treatment (T) | 1 | 1.24 | .30 | Treatment (T) | 1 | 1.81 | .18 | 1.91 | .17 | 0.03 | .85 | 0.08 | .77 | 0.00 | .98 |
| G × Gen | 6 | 6.08 | 3.067e‐15*** | G × Gen | 6 | 66.16 | 2.501e‐12*** | 59.61 | 5.394e‐11*** | 22.31 | .001066** | 27.59 | .0001124*** | 11.66 | .07 |
| G × T | 2 | 3.03 | .003557** | G × T | 2 | 10.83 | .0044538** | 8.50 | .01425* | 2.75 | .25 | 1.34 | .51 | 7.81 | .020177* |
| Gen × T | 3 | 2.61 | .002843** | Gen × T | 3 | 9.15 | .0273738* | 10.75 | .01314* | 3.24 | .36 | 1.34 | .72 | 11.64 | .008706** |
| G × Gen × T | 6 | 3.23 | 1.387e‐06*** | G × Gen × T | 6 | 11.75 | .07 | 38.13 | 1.061e‐06*** | 33.35 | 8.985e‐06*** | 1.33 | .97 | 0 | 1 |