Figure 3.

Routine PCR detection of gonadal differentially expressed genes in the HPG axis and muscle tissue and the structural difference of transcripts in one gene. (A) The expression of gonadal differentially expressed genes in the HPG axis and muscle tissue was detected by routine PCR. B, H, P, G, and M denote the brain, hypothalamus, pituitary, gonads, and muscle, respectively; N represents the negative control. (B) Schematic diagram of the genomic structure of two transcripts of LG24G003870 gene in the CDS region. The orange box indicates the missing exon in CDS2 that causes premature termination of translation.