. 2022 Jul 13;27(14):4468. doi: 10.3390/molecules27144468

Table 2.

List of independent variables and responses used in the design.

Independent Variables	Levels			Response	Constrains
Independent Variables	−1	0	+1	Response	Constrains
TPP concentration (mg/mL)	0.1	0.35	0.6	Particle size	Minimize
TPP concentration (mg/mL)	0.1	0.35	0.6	PDI	<0.5
CS concentration (mg/mL)	0.3	0.65	1	Zeta potential	30–40 mV
CS concentration (mg/mL)	0.3	0.65	1	Encapsulation efficiency	Maximize
Ultra-sonication energy (watt)	10	25	40	Release type	Sustained Release