Figure 3.

Alignment of cleavage site regions of all available newly emerged natural H5 and H7 MBCS-containing AIV sequences. Nucleotide and amino acid alignments of H5 MBCS-containing AIVs (A) and alignments of H7 MBCS-containing AIVs that did (B) or presumably did not (C) arise through NHR. The asterisks indicate strains that might have emerged through NHR based on codon usage and sequence alignment. The grey boxes delineate conserved amino acids from the LPAI consensus sequence with P1 to P4 indicated on top. Arginines and lysines are depicted in red and all other amino acids are depicted in blue. All sequences are available in fasta format as Supplementary Material.