TABLE 2.
Identities of clones related to numbered bands in Fig. 5, as determined by partial or nearly complete 16S rDNA sequencing
| Band | Accession no. | Closest relative in GenBank database (accession no.) | % Similarity | Phylogenetic group |
|---|---|---|---|---|
| 1 | AY013676a | Desulfosporosinus sp. strain S10 (AF07527) | 96 | Low-G+C-content gram-positive bacteria |
| 2 | AY013696ab | Gallionella ferruginea (L07897) | 94 | β-Proteobacteria |
| AY013693b | Uncultured Duganella sp. strain CTHB-18 (AF067655) | 93 | β-Proteobacteria | |
| 3 | AY013670 | Unidentified β-proteobacterium cda-1 (Y17060) | 96 | β-Proteobacteria |
| AY013688 | Unidentified β-proteobacterium cda-1 (Y17060) | 96 | β-Proteobacteria | |
| AY013694ab | Gallionella ferruginea (L07897) | 95 | β-Proteobacteria | |
| AY013698ab | Gallionella ferruginea (L07897) | 93 | β-Proteobacteria | |
| AY013691b | Actinomyces sp. (X92701) | 96 | High-G+C-content gram-positive bacteria | |
| AY013663 | Uncultured bacterium RB25 (Z95718) | 88 | Unclassified | |
| 4 | AY013697ab | Gallionella ferruginea (L07897) | 92 | β-Proteobacteria |
| AY013695b | Unidentified bacterium BD4-9 (AB015559) | 88 | Unclassified | |
| 5 | AY013690a | Azoarcus sp. strain BS5–8 (AF011350) | 93 | β-Proteobacteria |
| 6 | AY013666a | Azoarcus sp. strain BS5–8 (AF011350) | 93 | β-Proteobacteria |
| 7 | AY013669a | Azoarcus sp. strain BS5–8 (AF011350) | 94 | β-Proteobacteria |
| AY013681a | Azoarcus sp. strain BS5–8 (AF011350) | 94 | β-Proteobacteria | |
| 8 | AY013674 | Unidentified bacterium DGGE band 10 (AJ009652) | 98 | High-G+C-content gram-positive bacteria |
| AY013684a | Azoarcus sp. strain BS5–8 (AF011350) | 98 | High-G+C-content gram-positive bacteria | |
| AY013664a | Denitrifying bacterium 72Chol (Y09967) | 95 | β-Proteobacteria | |
| AY013675a | Azoarcus sp. strain BS5–8 (AF011350) | 93 | β-Proteobacteria | |
| AY013689 | Uncultured bacterium t0.6.f (AF005745) | 91 | Green nonsulfur bacteria | |
| AY013682 | Candidate division OP11 clone OPd29 (AF047561) | 90 | Unclassified | |
| 9 | AY013665a | Desulfovibrio aminophilus (AF067964) | 93 | δ-Proteobacteria |
| 10 | AY013593 | Acetobacterium carbonolicum (X96956) | 98 | Low-G+C-content gram-positive bacteria |
| AY013613 | Acetobacterium wieringae (X96955) | 97 | Low-G+C-content gram-positive bacteria | |
| AY013610b | Acetobacterium malicum (X96957) | 97 | Low-G+C-content gram-positive bacteria | |
| AY013607b | Acetobacterium malicum (X96957) | 95 | Low-G+C-content gram-positive bacteria | |
| 11 | AY013591 | Acetobacterium wieringae (X96955) | 98 | Low-G+C-content gram-positive bacteria |
| 12 | AY013609ab | Geobacter akaganeitreducens (U96918) | 94 | δ-Proteobacteria |
| 13 | AF013603 | Uncultured eubacterium WCHB1–21 (AF505080) | 96 | Low-G+C-content gram-positive bacteria |
| 14 | AY013658 | Uncultured freshwater bacterium (AF109142) | 98 | Unclassified |
| 15 | AY013644ab | Geobacter sp. strain CdA-2 (Y19190) | 96 | δ-Proteobacteria |
| 16 | AY013634a | Metal-contaminated soil clone K20-06 (AF145810) | 98 | δ-Proteobacteria |
| AY013642a | Metal-contaminated soil clone K20-06 (AF145810) | 95 | δ-Proteobacteria | |
| AY013647ab | Geobacter sp. strain CdA-2 (Y19190) | 96 | δ-Proteobacteria | |
| AY013648ab | Geobacter sp. strain CdA-2 (Y19190) | 94 | δ-Proteobacteria | |
| AY013651a | Geobacter sp. strain CdA-3 (Y13131) | 93 | δ-Proteobacteria | |
| AY013641a | Azoarcus sp. PCR strain (X85434) | 93 | δ-Proteobacteria | |
| AY013649b | Uncultured bacterium WCHB1–60 (AF050598) | 91 | Candidate division WS5 | |
| 17 | AY013652/3ac | Geobacter sp. strain (GSPY19190) | 96 | δ-Proteobacteria |
| 18 | AY013646b | Acidovorax sp. strain UFZ-B517 (AF235010) | 96 | δ-Proteobacteria |
| AY013643b | Rhodoferax fermentans (D16211) | 96 | δ-Proteobacteria | |
| AY013650b | Acidovorax devluvii (Y18616) | 91 | δ-Proteobacteria | |
| 19 | AY013645ab | Geobacter sp. strain CdA-2 (Y19190) | 95 | δ-Proteobacteria |
| AY013633 | Eubacterium limosum (M59120) | 94 | Low-G+C-content gram-positive bacteria | |
| AY013638 | Uncultured bacterium clone H1.4.f (AF005748) | 93 | Green nonsulfur bacteria | |
| 20 | AY013620a | Uncultured sulfate-reducing bacterium 368 (AJ389629) | 91 | δ-Proteobacteria |
| 21 | AY013625 | Uncultured clone CRE-FL35 (AF141457) | 97 | β-Proteobacteria |
a
The identity of the closest relative in the GenBank database gives an indication of the ability to perform redox reactions (microaerophilic, denitrification, iron reduction, or sulfate reduction).
b
The 16S rDNA was almost completely sequenced.
c
E. coli positions 8 to 518 and 1002 to 1512 were sequenced.