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. 2022 Jul 1;14(7):1461. doi: 10.3390/v14071461

Figure 2.

Figure 2

Characterization of disulfide-bonded RBD protein synthesized by wheat germ cell-free production system. (A) Antigenicity of disulfide-bonded RBD protein produced by wheat germ cell-free protein synthesis system with ERO1α and PDI (EP-WG). Each protein was incubated with or without 100 mM of DTT for 30 min at 37 °C and then subjected to ELISA using pre- or post-vaccinated serum (4 weeks after vaccination). The commercially available RBD protein produced by the mammalian cell expression system was used as a positive control (PC). The receiver operator characteristic (ROC) curves were constructed and the areas under the curves (AUCs) were calculated. (B) The KD of each RBD against ACE2. Biotinylated ACE2 was immobilized on the streptavidin biosensor and then analyzed for association with each RBD protein at various concentrations (1600, 800, 400, 200 and 100 nM for WG, and 400, 200, 100, 50 and 25 nM for EP-WG and PC) by OctetRED96 instrument.