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. 2022 Jul 26;17(7):e0270584. doi: 10.1371/journal.pone.0270584

Fig 5. Granzyme K induces chemokine release from A549 cells.

Fig 5

A549 cells were treated overnight with a serial dilution of either GzmA or GzmK in serum free medium and cell free supernatants were assayed for either (A) IL-6, or (B) IL-8 by ELISA. (C) A549 cells were pre-treated with inhibitors of PAR1 (vorapaxar), PAR2 (GB88) or MEK signalling (PD184352), then treated with either GzmK (white bars), GzmK-AI (grey bars) or PAR2 activating peptide (black bars) overnight in serum free medium. IL-8 release was measured by ELISA from cell-free supernatants. ****, p < 0.0001; ns, p > 0.05.