TABLE 2.

Influence of treatment of pP₂GMⁱⁱ and pP₂₅GMⁱⁱ with exo-β-d-galactofuranosidase (exo-β-Gal_fase) on the DP of galactofuran chains^a

Phosphate and carbohydrate components of pPGMx analyzed	Molar ratio for component pairs of the following polymers after the indicated treatment:
	pP25GMii		pP2GMii
	None	Exo-β-Galfasea	None	Exo-β-Galfase
Galactose/total carbohydrate	0.55	0.37	0.60	0.16
Galactose/nrtGalactose	6.0	3.7	5.4	1.3
Total carbohydrate/PO4	10	9.2	166	109
Galactofuran chains/mol	23	18	32	17

^aReaction mixtures with 4 to 8 μmol of galactofuranosyl residues from pP₂GMⁱⁱ (50 kDa) or pP₂₅GMⁱⁱ (47 kDa) in 1.5 ml of 66 mM acetate buffer (pH 4.0) containing 6 μg of purified enzyme were incubated in a 1-dm cuvette in a JASCO DIP polarimeter at room temperature for 200 or 130 h, respectively. Galactose was removed through a Spectrapor membrane. The retentate was analyzed for total phenol-sulfuric acid-positive carbohydrate, galactose, nonreducing terminal galactofuranosyl residues (nrtGalactose), and phosphate. Similar untreated samples of polymers served as controls.