Table 2.
Predicted ORFs in the genome of phage BUCT610.
| ORF | Strand | Start | Stop | Length (AA) |
Putative Function | Best-Match BLASTp Result | Query Cover (%) | E-Values | Identity (%) | Accession | MW (kDa) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| ORF1 | - | 352 | 20 | 110 | hypothetical protein | Klebsiella phage KMI7 | 98% | 7.00E-41 | 62.04% | QEG10317.1 | 13.14 |
| ORF2 | - | 729 | 439 | 96 | hypothetical protein | Klebsiella phage YX3973 | 100% | 2.00E-62 | 98.96% | YP_010054377.1 | 10.70 |
| ORF3 | + | 818 | 1057 | 79 | hypothetical protein | Klebsiella phage ZX4 | 100% | 3.00E-48 | 97.47% | YP_010054495.1 | 9.32 |
| ORF4 | + | 1054 | 1206 | 50 | hypothetical protein | Trabulsiella odontotermitis | 96% | 1.00E-06 | 50.00% | WP_158674504.1 | 5.67 |
| ORF5 | + | 1215 | 1394 | 59 | hypothetical protein | Salmonella phage SeSz-2 | 100% | 1.00E-14 | 61.02% | YP_010053632.1 | 6.84 |
| ORF6 | + | 1394 | 1690 | 98 | hypothetical protein | Pectinobacterium phage PEAT2 | 95% | 1.00E-37 | 63.83% | YP_009702196.1 | 11.68 |
| ORF7 | + | 1687 | 1875 | 62 | hypothetical protein | Escherichia coli | 100% | 4.00E-24 | 70.97% | MBB0589830.1 | 6.70 |
| ORF8 | + | 1875 | 2075 | 66 | hypothetical protein | Salmonella enterica subsp | 100% | 4.00E-07 | 34.85% | HAZ2983796.1 | 7.66 |
| ORF9 | + | 2085 | 2597 | 170 | hypothetical protein | Salmonella enterica | 97% | 6.00E-64 | 57.74% | EGT9551729.1 | 19.34 |
| ORF10 | + | 2646 | 4094 | 482 | portal protein | Klebsiella phage ZX4 | 100% | 0 | 97.30% | YP_010054497.1 | 54.12 |
| ORF11 | + | 4075 | 5019 | 314 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 0 | 97.13% | YP_010054477.1 | 35.56 |
| ORF12 | - | 5226 | 5032 | 64 | hypothetical protein | Klebsiella phage YX3973 | 100% | 5.00E-31 | 82.81% | YP_010054385.1 | 7.53 |
| ORF13 | - | 6049 | 5228 | 273 | hypothetical protein | Klebsiella phage YX3973 | 100% | 5.00E-91 | 56.12% | YP_010054386.1 | 30.50 |
| ORF14 | - | 6177 | 6046 | 43 | hypothetical protein | Klebsiella phage ZX4 | 100% | 3.00E-21 | 93.02% | YP_010054501.1 | 5.12 |
| ORF15 | - | 6467 | 6177 | 96 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 1.00E-57 | 94.79% | YP_010054472.1 | 9.95 |
| ORF16 | + | 6566 | 7003 | 145 | TPA: lysozyme | Salmonella enterica subsp | 100% | 3E-103 | 97.93% | HAZ2983869.1 | 15.70 |
| ORF17 | + | 7000 | 7245 | 81 | hypothetical protein | K. pneumoniae | 100% | 3.00E-51 | 100.00% | WP_142483241.1 | 9.06 |
| ORF18 | + | 7220 | 7717 | 165 | DUF2514 family protein | Klebsiella virus KpV2811 | 96% | 3.00E-91 | 93.71% | YP_010054469.1 | 18.13 |
| ORF19 | - | 8013 | 7864 | 49 | hypothetical protein | K. pneumoniae | 100% | 5.00E-25 | 95.92% | WP_185939371.1 | 5.64 |
| ORF20 | - | 8233 | 8021 | 70 | hypothetical protein | Klebsiella phage YX3973 | 100% | 1.00E-40 | 91.43% | YP_010054391.1 | 8.20 |
| ORF21 | - | 8700 | 8233 | 155 | hypothetical protein | Vibrio phage pYD38-A | 100% | 7.00E-91 | 86.16% | YP_008126189.1 | 17.50 |
| ORF22 | - | 8900 | 8700 | 66 | hypothetical protein | K. pneumoniae | 100% | 2.00E-40 | 100.00% | WP_142483236.1 | 8.02 |
| ORF23 | + | 8997 | 9212 | 71 | hypothetical protein | Klebsiella phage YX3973 | 100% | 2.00E-44 | 100.00% | YP_010054394.1 | 8.26 |
| ORF24 | + | 9296 | 9487 | 63 | hypothetical protein | K. pneumoniae | 100% | 1.00E-35 | 96.83% | WP_142483234.1 | 7.70 |
| ORF25 | + | 9484 | 9723 | 79 | hypothetical protein | Vibrio phage pYD38-A | 100% | 6.00E-35 | 89.87% | YP_008126184.1 | 9.62 |
| ORF26 | + | 9704 | 9946 | 80 | hypothetical protein | Salmonella phage SeSz-2 | 97% | 8.00E-28 | 66.67% | YP_010053654.1 | 8.97 |
| ORF27 | + | 9936 | 10,406 | 156 | hypothetical protein | Klebsiella phage YX3973 | 100% | 8E-113 | 100.00% | YP_010054398.1 | 17.67 |
| ORF28 | + | 10,394 | 10,819 | 141 | hypothetical protein | Shigella phage Sf11 SMD-2017 | 55% | 1.00E-39 | 80.77% | YP_010053088.1 | 16.52 |
| ORF29 | + | 10,816 | 11,160 | 167 | hypothetical protein | Klebsiella phage YX3973 | 68% | 8.00E-74 | 93.86% | YP_010054400.1 | 13.05 |
| ORF30 | + | 11,343 | 12,746 | 467 | hypothetical protein | Klebsiella phage YX3973 | 100% | 0 | 96.15% | YP_010054401.1 | 51.39 |
| ORF31 | + | 12,746 | 13,210 | 207 | hypothetical protein | Vibrio phage pYD38-A | 74% | 7E-103 | 97.40% | YP_008126178.1 | 15.64 |
| ORF32 | + | 13,222 | 14,310 | 362 | DUF2184 domain containing protein |
K. pneumoniae | 100% | 0 | 99.45% | WP_142483227.1 | 40.05 |
| ORF33 | - | 15,123 | 14,326 | 265 | hypothetical protein | Salmonella enterica | 100% | 5E-116 | 60.97% | ECD2278621.1 | 31.84 |
| ORF34 | + | 15,215 | 15,409 | 64 | hypothetical protein | Vibrio phage pYD38-A | 100% | 6.00E-37 | 98.44% | YP_008126175.1 | 7.11 |
| ORF35 | + | 15,453 | 16,271 | 272 | hypothetical protein | K. pneumoniae | 94% | 1E-180 | 97.66% | WP_142483225.1 | 28.65 |
| ORF36 | - | 16,483 | 16,301 | 60 | hypothetical protein | Salmonella phage IME207 | 100% | 2.00E-28 | 83.33% | YP_009322759.1 | 6.76 |
| ORF37 | - | 16,682 | 16,509 | 57 | hypothetical protein | K. pneumoniae | 100% | 4.00E-33 | 96.49% | WP_185939376.1 | 6.71 |
| ORF38 | - | 16,819 | 16,682 | 45 | hypothetical protein | Bacteriophage sp. | 97% | 3.00E-15 | 68.18% | QHJ81118.1 | 5.27 |
| ORF39 | - | 17,634 | 16,819 | 271 | hypothetical protein | K. pneumoniae | 100% | 0 | 98.52% | WP_142483222.1 | 29.94 |
| ORF40 | - | 17,758 | 17,642 | 38 | hypothetical protein | Klebsiella phage ZX4 | 100% | 5.00E-14 | 92.11% | YP_010054525.1 | 4.42 |
| ORF41 | - | 18,048 | 17,755 | 97 | hypothetical protein | Vibrio phage pYD38-A | 100% | 6.00E-22 | 53.61% | YP_008126169.1 | 10.02 |
| ORF42 | + | 18,279 | 18,566 | 95 | hypothetical protein | Klebsiella phage YX3973 | 100% | 5.00E-62 | 100.00% | YP_010054411.1 | 10.16 |
| ORF43 | + | 18,544 | 18,936 | 130 | hypothetical protein | Klebsiella phage YX3973 | 100% | 9.00E-89 | 97.69% | YP_010054412.1 | 13.88 |
| ORF44 | + | 18,974 | 19,399 | 141 | hypothetical protein | Vibrio phage pYD38-A | 80% | 8.00E-79 | 100.00% | YP_008126166.1 | 15.18 |
| ORF45 | + | 19,504 | 19,920 | 138 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 5.00E-94 | 98.55% | YP_010054440.1 | 14.99 |
| ORF46 | + | 19,917 | 20,300 | 127 | hypothetical protein | Vibrio phage pYD38-A | 100% | 6.00E-89 | 100.00% | YP_008126240.1 | 14.47 |
| ORF47 | - | 20,509 | 20,324 | 61 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 1.00E-35 | 95.08% | YP_010054438.1 | 7.06 |
| ORF48 | + | 20,713 | 21,015 | 100 | hypothetical protein | Pectobacterium polonicum | 39% | 9.00E-09 | 82.05% | WP_137741933.1 | 11.10 |
| ORF49 | + | 21,219 | 21,974 | 251 | hypothetical protein | K. pneumoniae | 99% | 2e-178 | 99.60% | WP_142483279.1 | 26.54 |
| ORF50 | - | 22,298 | 21,996 | 100 | hypothetical protein | Salmonella enterica | 100% | 5.00E-55 | 81.00% | EAQ4012616.1 | 12.12 |
| ORF51 | - | 22,450 | 22,298 | 50 | hypothetical protein | Klebsiella phage ZX4 | 100% | 1.00E-25 | 94.00% | YP_010054538.1 | 5.74 |
| ORF52 | - | 22,817 | 22,440 | 125 | DUF2591 family protein | K. pneumoniae | 96% | 2.00E-44 | 63.11% | WP_116961239.1 | 13.94 |
| ORF53 | - | 22,948 | 22,814 | 44 | hypothetical protein | Klebsiella phage ZX4 | 95% | 3.00E-17 | 88.10% | YP_010054539.1 | 5.15 |
| ORF54 | - | 23,244 | 22,945 | 99 | hypothetical protein | Vibrio phage pYD38-A | 100% | 4.00E-60 | 92.93% | YP_008126235.1 | 10.99 |
| ORF55 | + | 23,338 | 24,045 | 235 | hypothetical protein | Klebsiella phage ZX4 | 100% | 7E-171 | 97.45% | YP_010054540.1 | 26.55 |
| ORF56 | + | 24,045 | 27,338 | 1097 | tail length tape measure protein | Vibrio phage pYD38-A | 100% | 0 | 96.08% | YP_008126233.1 | 114.54 |
| ORF57 | + | 27,338 | 27,808 | 156 | hypothetical protein | K. pneumoniae | 100% | 3E-112 | 100.00% | WP_142483272.1 | 17.99 |
| ORF58 | + | 27,808 | 28,278 | 156 | hypothetical protein | Klebsiella phage YX3973 | 100% | 1E-109 | 98.72% | YP_010054357.1 | 17.93 |
| ORF59 | + | 28,241 | 28,699 | 152 | C40 family peptidase | K. pneumoniae | 100% | 2E-107 | 98.03% | WP_142483270.1 | 17.40 |
| ORF60 | + | 28,671 | 31,127 | 818 | hypothetical protein | Vibrio phage pYD38-A | 100% | 0 | 99.02% | YP_008126229.1 | 89.86 |
| ORF61 | + | 31,166 | 33,430 | 754 | hypothetical protein | K. pneumoniae | 95% | 0 | 66.02% | WP_191974704.1 | 80.67 |
| ORF62 | + | 33,427 | 33,672 | 81 | hypothetical protein | K. pneumoniae | 96% | 8.00E-19 | 47.06% | WP_211775938.1 | 9.06 |
| ORF63 | - | 34,175 | 33,702 | 157 | single-strandedDNA-binding protein | Vibrio phage pYD38-A | 100% | 9.00E-48 | 52.10% | YP_008126226.1 | 17.65 |
| ORF64 | - | 34,844 | 34,185 | 219 | recombinase | Klebsiella phage ZX4 | 100% | 1E-154 | 98.17% | YP_010054548.1 | 23.76 |
| ORF65 | - | 35,824 | 34,868 | 318 | exonuclease | Klebsiella virus KpV2811 | 100% | 0 | 97.80% | YP_010054422.1 | 36.00 |
| ORF66 | - | 36,012 | 35,824 | 62 | hypothetical protein | Vibrio phage pYD38-A | 100% | 4.00E-37 | 98.39% | YP_008126223.1 | 7.13 |
| ORF67 | - | 36,475 | 36,059 | 138 | hypothetical protein | Vibrio phage pYD38-A | 100% | 5.00E-95 | 94.93% | YP_008126222.1 | 15.74 |
| ORF68 | - | 38,504 | 36,462 | 680 | DNA helicase | Klebsiella virus KpV2811 | 100% | 0 | 95.15% | YP_010054420.1 | 76.95 |
| ORF69 | + | 38,579 | 39,472 | 297 | hypothetical protein | K. pneumoniae | 100% | 0 | 93.94% | WP_142483294.1 | 33.36 |
| ORF70 | - | 39,783 | 39,487 | 98 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 7.00E-62 | 90.82% | YP_010054418.1 | 11.46 |
| ORF71 | - | 41,336 | 39,783 | 517 | hypothetical protein | Klebsiella phage ZX4 | 100% | 0 | 99.81% | YP_010054556.1 | 57.52 |
| ORF72 | - | 41,707 | 41,333 | 124 | hypothetical protein | Aeromonas phage pIS4-A | 87% | 6.00E-59 | 87.04% | YP_009614674.1 | 13.87 |
| ORF73 | + | 42,064 | 42,285 | 73 | hypothetical protein | Vibrio phage pYD38-A | 100% | 4.00E-40 | 90.41% | YP_008126215.1 | 8.05 |
| ORF74 | + | 42,282 | 42,467 | 61 | hypothetical protein | Vibrio phage pYD38-A | 100% | 3.00E-36 | 96.72% | YP_008126214.1 | 6.76 |
| ORF75 | + | 42,495 | 42,761 | 88 | hypothetical protein | Klebsiella virus KpV2811 | 100% | 4.00E-55 | 98.86% | YP_010054492.1 | 10.03 |
| ORF76 | + | 42,873 | 43,220 | 115 | hypothetical protein | Klebsiella | 99% | 9.00E-50 | 74.56% | WP_004151294.1 | 12.92 |
| ORF77 | + | 43,217 | 43,615 | 132 | hypothetical protein | K. pneumoniae | 100% | 2.00E-94 | 99.24% | WP_117033037.1 | 15.28 |
| ORF78 | + | 43,618 | 44,235 | 205 | DUF551 domain-containing | Escherichia coli | 59% | 2.00E-60 | 81.60% | EFM1806727.1 | 22.84 |
| ORF79 | + | 44,328 | 44,486 | 52 | hypothetical protein | Klebsiella phage YX3973 | 100% | 9.00E-29 | 92.31% | YP_010054372.1 | 6.06 |
| ORF80 | + | 44,486 | 44,680 | 64 | hypothetical protein | Klebsiella phage YX3973 | 100% | 4.00E-38 | 95.31% | YP_010054373.1 | 7.10 |
| ORF81 | + | 44,784 | 45,203 | 139 | hypothetical protein | K. pneumoniae | 99% | 5.00E-92 | 99.28% | WP_142483254.1 | 15.55 |
| ORF82 | + | 45,196 | 45,351 | 51 | hypothetical protein | NO hit | - | - | - | - | 5.58 |
| ORF83 | + | 45,341 | 46,762 | 473 | large terminase subunit | Salmonella phage SeSz-2 | 100% | 0 | 89.64% | YP_010053627.1 | 54.99 |