Table 1.
Gene Name | Primers Sequences (5′-3′) | Product Size (bp) | Annealing Temperature | Accession Number |
---|---|---|---|---|
SIRT1 | Forward: GCTCGCCTTGCTGTGGACTTCC Reverse: GTGACACAGAGATGGCTGGAACTG |
141 | 60 °C | NM_001372090.1 |
PGC-1α | Forward: CATTCAGGAGCTGGATGGCT Reverse: AGATCTGGGCAAAGAGGCTG |
106 | 60 °C | NM_031347.1 |
Nrf1 | Forward: GGCGCAGCACCTTTGGAGAATGTG Reverse: CATCGATGGTGAGAGGGGGCAGTTC |
133 | 60 °C | NM_001100708.1 |
Nrf2 | Forward: GAGACGGCCATGACTGA Reverse: GTGAGGGGATCGATGAGTAA |
196 | 60 °C | NM_031789.2 |
TNF-α | Forward: CCACGCTCTTCTGTCTACTG Reverse: GCTACGGGCTTGTCACTC |
145 | 60 °C | NM_012675.3 |
IL-1β | Forward: TCTGTGACTCGTGGGATGAT Reverse: CTTCTTTGGGTATTGTTTGG |
181 | 60 °C | NM_031512.2 |
β-actin | Reverse: CCCAGGCATTGCTGACAGGATG Forward: TGCTGGAAGGTGGACAGTGAGG |
144 | 60 °C | NM_031144.3 |