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. 2022 Jul 21;23(14):8042. doi: 10.3390/ijms23148042

Table 1.

Primer sequences for quantitative RT-PCR analyses.

Gene Name Primers Sequences (5′-3′) Product Size (bp) Annealing Temperature Accession
Number
SIRT1 Forward: GCTCGCCTTGCTGTGGACTTCC
Reverse: GTGACACAGAGATGGCTGGAACTG
141 60 °C NM_001372090.1
PGC-1α Forward: CATTCAGGAGCTGGATGGCT
Reverse: AGATCTGGGCAAAGAGGCTG
106 60 °C NM_031347.1
Nrf1 Forward: GGCGCAGCACCTTTGGAGAATGTG
Reverse: CATCGATGGTGAGAGGGGGCAGTTC
133 60 °C NM_001100708.1
Nrf2 Forward: GAGACGGCCATGACTGA
Reverse: GTGAGGGGATCGATGAGTAA
196 60 °C NM_031789.2
TNF-α Forward: CCACGCTCTTCTGTCTACTG
Reverse: GCTACGGGCTTGTCACTC
145 60 °C NM_012675.3
IL-1β Forward: TCTGTGACTCGTGGGATGAT
Reverse: CTTCTTTGGGTATTGTTTGG
181 60 °C NM_031512.2
β-actin Reverse: CCCAGGCATTGCTGACAGGATG
Forward: TGCTGGAAGGTGGACAGTGAGG
144 60 °C NM_031144.3