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Single and dual staining of heat-treated seawater samples. Panels: D1 and D2, heat-treated seawater samples supplemented with PI and SYBR Green II, respectively; D3, heat-treated (40 min at 60 to 70°C) seawater sample simultaneously stained with PI and SYBR Green. Killed bacteria essentially appeared as red fluorescent cells. The final probe concentrations were 10 μg of PI cm−3 and 1:1,000 (vol/vol) SYBR Green II.
