Skip to main content
. 2022 Apr 30;600(12):2853–2875. doi: 10.1113/JP282828

Figure 1. The molecular players of nerve growth factor signalling predominantly localize at the neuro‐cardiac junction, in rat hearts.

Figure 1

A , confocal immunofluorescence (IF) analysis of heart sections from adult rats, co‐stained with antibodies against SNAP25 (left panel) and TrkA (middle panel). The right panel shows the bright field images (BF). SN, sympathetic neuron; CM, cardiomyocyte. The white arrow indicates the neuronal process. B, confocal IF analysis of adult rat heart sections, co‐stained with antibodies against SNAP25 (left panel) and nerve growth factor (NGF) (middle panel). The right panel shows merged fluorescence and bright field images. The white arrow indicates the SN process. C, magnified image of panel B. D, surface rendering of the z‐section series of the IF images shown in (B) and relative quantification of the fluorescence intensity of SNAP25 (red signal) and NGF (green signal) in correspondence with the neuronal varicosity (2) or the inter‐varicosity space (1). Bars represent SD (n = 66 neuro‐cardiac contacts from three different rat hearts). [Colour figure can be viewed at wileyonlinelibrary.com]