TABLE 1.
PCR primers used for amplifying the LmPH fragments
| Primera | Nucleotide sequenceb | Positionsc | Specificity |
|---|---|---|---|
| phe1f | GA(G/A)GGCATCAA(A/G)AT(C/T) | 121–134 | Universal |
| phe2f | CC(C/T/G)TTCATGTC(C/G)GG(T/A/C)GC | 628–644 | Universal |
| phe3r | CAG(C/G)CG(A/G)T(A/T)ACC(G/T)CGCCAGAACC | 797–819 | Universal |
| phe4r | AT(C/T)TG(G/A)TGCAC(C/A)GGCA(G/A)CC | 1355–1373 | Universal |
| pheUf | CCAGG(C/G)(C/G/T)GA(G/A)AA(A/G)GAGA(A/G)GAA(G/A)CT | 195–218 | Universal |
| pheUr | CGG(A/T)A(G/A)CCGCGCCAGAACCA | 796–815 | Universal |
| pheLr | GG(A/G/C)A(G/T/C)(A/G)TTG(C/T)CCGGGTC | 757–774 | Group I |
| pheMHr | GAT(T/C/G)GGCAC(A/G)TTGTCTTC | 760–777 | Groups II and III |
| pheHr | GTGGCCATGTCGCCATTGA | 660–668 | Group III |
a
f, forward primer; r, reverse primer.
b
Degenerate nucleotides are indicated in parentheses.
c
Corresponding to the dmpN sequence of Pseudomonas sp. strain CF600.