TABLE 2.
Characteristics of isolated strains
| Isolate | Closely related organism based on 16S rRNA gene sequence (% identity) | Rep-PCR pattern no.a | Group-specific PCRb with primers:
|
Mean kl ± SD (liter g−1 h−1)c | |||
|---|---|---|---|---|---|---|---|
| Universal | Group I | Group II | Group III | ||||
| HAB-01 | Ralstonia sp. strain BKME-6 (99) | 1 | + | + | − | − | 18 ± 0.5 |
| HAB-02 | Ralstonia sp. strain BKME-6 (99) | 2 | + | + | − | − | 18 ± 0.5 |
| HAB-08 | Ralstonia eutropha KT-1 (99) | 3 | + | + | − | − | NA |
| HAB-11 | Ralstonia eutropha KT-1 (99) | 4 | + | + | − | − | 22 ± 3.5 |
| HAB-18 | Ralstonia eutropha KT-1 (99) | 5 | + | + | − | − | 32 ± 2.7 |
| HAB-21 | Variovorax sp. strain WFF52 (99) | 6 | + | + | − | − | 24 ± 0.8 |
| HAB-22 | Variovorax sp. strain WFF52 (99) | 7 | + | + | − | − | ND |
| HAB-23 | Variovorax sp. strain WFF52 (99) | 7 | + | + | − | − | NA |
| HAB-24 | Variovorax sp. strain WFF52 (99) | 8 | + | + | − | − | 98 ± 0.3 |
| HAB-27 | Variovorax sp. strain WFF52 (99) | 9 | + | + | − | − | 99 ± 5.8 |
| HAB-29 | Variovorax sp. strain WFF52 (99) | 9 | + | + | − | − | 91 ± 4.6 |
| HAB-30 | Variovorax sp. strain WFF52 (99) | 10 | + | + | − | − | 200 ± 6.7 |
| LAB-05 | Pseudomonas sp. strain IpA-2 (99) | 11 | + | − | + | + | NA |
| LAB-06 | Pseudomonas sp. strain BKME-9 (98) | 12 | + | − | + | + | 1.7 ± 0.2d |
| LAB-08 | Pseudomonas sp. strain DhA-51 (99) | 13 | + | − | + | + | NA |
| LAB-16 | Pseudomonas sp. strain PsF (99) | 14 | + | − | + | + | NA |
| LAB-18 | Pseudomonas sp. strain DhA-51 (99) | 15 | + | − | − | + | 1.4 ± 0.04d |
| LAB-20 | Pseudomonas sp. strain IpA-2 (99) | 16 | + | − | + | + | 0.3 ± 0.2 |
| LAB-21 | Pseudomonas putida ATCC 17484 (99) | 11 | + | − | + | + | 1.3 ± 0.04d |
| LAB-23 | Pseudomonas sp. strain PsF (99) | 17 | + | − | + | + | 1.3 ± 0.18 |
| LAB-26 | Pseudomonas jessenii (99) | 18 | + | − | + | + | 1.0 ± 0.05 |
| LAB-27 | Pseudomonas sp. strain DhA-51 (99) | 19 | + | − | + | + | ND |
| LAB-36 | Pseudomonas agarici (97) | 20 | + | − | + | + | 0.3 ± 0.1 |
| LAB-44 | Pseudomonas rhodesiae (100) | 21 | + | − | + | + | 1.4 ± 0.06d |
a
Identical numbers indicate identical rep-PCR patterns.
b
+, fragment of the expected size amplified; −, fragment of the expected size not amplified.
c
The values were determined using cells grown in chemostat cultures as described previously (8); data are means for groups of three. NA, not assayed; ND, not detected.
d
Value determined using cells grown in batch culture and harvested at the late exponential growth phase.