Table 2.
Sequence of the specific primers used for cloning and quantitative RT‐PCR (qPCR) mRNA expression analysis. Sequence accession number, primer sequences, amplicon sizes, R 2 and qPCR efficiency are indicated for each primer pair
| Gene | GenBank Acc. No. | Sequence (5’→3’) | Amplicon (bp) | R 2 | Efficiency (%) |
|---|---|---|---|---|---|
| Cloning | |||||
| slc15a2a | XM_01 416 5384.1 | F: ATGGGGAAACGAAAGGAGCTGA | 2166 | ||
| R: CTACATCTTGGTGCTCTTCTCTATT | |||||
| slc15a2b | XM_01 417 3652.1 | F: ATGGAGACACGAAAGGAACATGA | 2187 | ||
| R: CTACATCTTAGTGCTCTTCTCTAT | |||||
| qPCR | |||||
| β‐actin | NM_0 011 23525.1 | F: CCAAAGCCAACAGGGAGAAG | 91 | 0.9997 | 99.89 |
| R: AGGGACAACACTGCCTGGAT | |||||
| slc15a2a | XM_01 416 5384.1 | F: GGGGGACACAACAAGACCAT | 198 | 0.9983 | 98.52 |
| R: CCGCGTGTTTATGAACCTCA | |||||
| slc15a2b | XM_01 417 3652.1 | F: GGATCAGTGGATGGAGTTCCTG | 170 | 0.9976 | 92.47 |
| R: TCTGGTTCTTTTTCAAGTTGTCTTC |