Figure 2.

iPSC differentiation into iBMECs. (A). Representative immunofluorescence staining, at d1 co-culture in transwell filters, demonstrating the expression of endothelial relevant proteins: vWF, ZO-1, PECAM1, and Claudin-5. Nuclei were counterstained with Hoechst (blue). Scale bar represented 50 µm. (B) LDL uptake (in red) by iBMECs (at D10). In green, the intracellular distribution of LDLR was also reported (scale bar indicates 20 µm).