Fig. 5.
Pseudomonas syringae pv tomato (Overexpression of NON‐RACE‐SPECIFIC DISEASE RESISTANCE1 (NDR1) in Arabidopsis results in enhanced RPM1‐interacting protein 4 (RIN4) stability in the presence of Pst)‐AvrRpt2. (a) Detection of RIN4 at 0, 2, 4, 6, and 8 h after syringe‐infiltration with Pst‐AvrRpt2 (OD600 nm = 0.1) in wild‐type (Col‐0) and mutant plants. The total protein extracts were subjected to ⍺‐RIN4 Western blot. Equal loading of protein was verified by ponceau S staining of the membrane after protein transfer. (b–e) Effector translocation in ndr1, ndr1/35S::NDR1, and ics1‐2, respectively, following syringe‐infiltration with Pst hrcC− or Pst‐expressing AvrRpt2‐CyaA (OD600 = 0.005). Tissue was collected at 0 and 10 h post‐inoculation (hpi) for quantification of cyclic adenosine monophosphate (cAMP) which was normalized by total protein. Higher levels of cAMP indicate more translocation of bacterial effectors. n represents the total number of leaves from three independent biological repeats (n = 6). Values are plotted as boxplots split by the median, and the whiskers show the range of data. Different letters represent a significant difference at P < 0.05 with Tukey’s honest significant difference (HSD) test. All data are representative of three independent experiments.