TABLE 3.

TaqMan probe and primers for the nonclassical hemolysin (hlyA) gene region are specific for detection of V. cholerae O1, O139, non-O1, and non-O139 species

Probe or primer	Sequence (5′→3′)	Denaturation temp (°C)a
hlyA primer
Forward	TGC GTT AAA CAC GAA GCG AT	58
Reverse	AAG TCT TAC ATT GTG CTT GGG TCA	59
TaqMan probe	TCA ACC GAT GCG ATT GCC CAA GA	69

^aCalculated by the nearest-neighbor algorithm with the Primer Express (PE Applied Biosystems) program.