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. 2022 Jul 8;14(7):1505. doi: 10.3390/v14071505

Figure 4.

Figure 4

Divergence in translation efficiency between the 5′UTRs of MERS-CoV and SARS-CoV gRNA. (A) Percentage of identity between the 5′UTRs of MERS-CoV, SARS-CoV-1 and SARS-CoV-2 gRNAs resulting from a MUSCLE alignment (Figure S2). (B) Scheme of capped and polyadenylated mRNAs coding for the Renilla reporter gene with the leader or the 5′UTRORF1ab from SARS-CoV-1/2 and MERS-CoV. (C) RRL was programmed with the corresponding in vitro transcribed reporter mRNAs. Expression products were quantified by luciferase assays and normalized to globin 5′UTR expression (set at 100%). (D) Jurkat cells were electroporated with capped and polyadenylated mRNAs as indicated. After 90 min of incubation, the cellular extracts were used for luciferase assay and RT-qPCR. All the results are normalized to the globin 5′UTR expression (set at 100%). Values shown are the mean (+/− S.E.M.) for 6 (C) and 5 (D) independent experiments.