Figure 3.

T-cell numbers in Babraham pigs. Inbred Babraham animals were inoculated with low virulent ASFV isolate OUR T88/3 (red and blue) or PBS control (green) and then challenged with highly virulent OUR T88/1 eighteen days later (arrows). Blood samples were taken on the indicated day, labelled with antibodies and the number of CD3+γδTCR-CD4+CD8α- (A), CD3+γδTCR-CD4+CD8α+ (B), CD3+γδTCR-CD4+CD25+ (C) and CD3+γδTCR-CD8α+CD4- (D) determined by volumetric flow cytometry. Animals immunised with OUR T88/3 that recovered after challenge are indicated as blue symbols and those that did not as dark red symbols, and individual animals are labelled as shown in the legend of Figure 2. Lines indicate the mean, and error bars indicate the standard deviation from that mean. * p < 0.05, ** p < 0.01, *** p < 0.001. Individual pigs are labelled as in Figure 2.