Figure 4.

Cellular responses to ASFV in Babraham pigs. Inbred Babraham animals were inoculated with low virulent ASFV isolate OUR T88/3 (red and blue) or PBS control (green) and then challenged with highly virulent OUR T88/1 eighteen days later. Blood samples were taken on the indicated day (A) or prior to challenge, 18 days post immunisation (B–F). IFNγ secreting cells were enumerated by ELISpot (A) or after labelling with antibodies for analysis by flow cytometry to identify CD8α+CD4- (B), CD8α+CD4-CD8β+ (C), CD4+CD8α+ (D), CD3-CD8α+ (E), or CD3+γδTCR+ cells (F). The proportion of each cell type expressing IFNγ, TNFα or both cytokines was determined using ICS (B–D). Animals immunised with OUR T88/3 that recovered after challenge are indicated as solid blue symbols and those that did not as open dark red symbols and control as green. Lines indicate the mean, and error bars indicate the standard deviation from that mean.