TABLE 1.
Molecular properties of the recombinantly expressed basidiomycete PhyA phytases
| Phytase | Length (aa)a | Predicted cleavage siteb between: | N-terminal sequencec | N-gly sitesd | Mr
|
pI
|
||
|---|---|---|---|---|---|---|---|---|
| Calculated | SDS-PAGE | Calculatede | IEF | |||||
| P. lycii PhyA | 439 | A29 and Q30 | L31PIPAQXTXN | 10 | 44,583 | 72,000 | 4.35 | 3.61 ± 0.01 |
| A. pediades PhyA | 453 | G19 and G20 | F31PPQIQDSXAAY | 6 | 46,781 | 59,000 | 5.00 | 4.15 ± 0.01 |
| 4.66 ± 0.01 | ||||||||
| 4.78 ± 0.01 | ||||||||
| 4.86 ± 0.01 | ||||||||
| Ceriporia sp. PhyA1 | 442 | A19 and T20 | S21VPKNTAPXFX | 7 | 45,942 | 59,000 | 6.40 | 7.36 ± 0.01 |
| 8.01 ± 0.03 | ||||||||
| Ceriporia sp. PhyA2 | 442 | A19 and A20 | N25IAPKFSIP | 8 | 44,955 | 54,000 | 4.64 | 3.94 ± 0.01 |
| 4.00 ± 0.01 | ||||||||
| 4.06 ± 0.01 | ||||||||
| 4.18 ± 0.01 | ||||||||
| T. pubescens PhyA | 443 | A19 and V20 | S31AXLDVTRDV | 9 | 44,531 | 62,000 | 4.40 | 3.58 ± 0.02 |
a
Including the signal sequence. aa, amino acids.
b
Signal peptide cleavage site predicted with the program SignalP, version 1.1 (17).
c
Determined from the purified recombinantly expressed phytases. The subscript number indicates the position of the first amino acid determined by Edman sequencing.
d
Number of potential N-glycosylation (N-gly) sites.
e
Calculated by using amino acid sequence of the mature protein (i.e., without signal sequence).