TABLE 2.
Characteristics of purified, recombinantly expressed phytases
| Phytase | Sp act (U/mg) | pH optimum | Temp optimum (°C) | Tm (°C) | % Residual activitya |
|---|---|---|---|---|---|
| P. lycii PhyA | 1,080 ± 110 | 4.0–4.5 | 50–55 | 60 | 62 |
| A. pediades PhyA | 400 | 5.0–6.0 | 50 | 58 | 47 |
| Ceriporia sp. PhyA1 | 700 ± 80 | 5.5–6.0 | 55–60 | 59 | 38 |
| Ceriporia sp. PhyA2 | 1,040 ± 310 | 5.0–6.0 | 40–45 | 48 | 22 |
| T. pubescens PhyA | 1,210 ± 30 | 5.0–5.5 | 50 | 55 | 15 |
| A. niger PhyA | 100b | 2.5–5.5b | 50b | 57 | 52 |
a
Residual activities were measured after preincubation of the enzymes for 60 min at 80°C in 0.1 M sodium acetate, pH 5.5.
b
R. F. M. van Gorcom, W. van Hartingsveldt, P. A. van Paridon, A. E. Veenstra, R. G. M. Luiten, and G. C. M. Selten, 3 April 1991, European Patent Application EP-0420358-A1.