Fig. 7.

The mRNA expression of key genes on days 11 and 24 post-surgery. The callus tissue was grounded into powder with liquid nitrogen, then total RNA was extracted using TRIzol reagent, and reverse transcribed to cDNA by M-MLV reverse transcriptase. The mRNA expression of Aggrecan, Col10a, VEGF, MMP13, Col1a and OCN were examined using real-time PCR. Data are presented as mean ± SD (n = 6 per group). Multiple comparisons were done using one way ANOVA analysis. ^a P < 0.05, ^aa P < 0.01 versus control group; ^b P < 0.05, ^bb P < 0.01 versus model group.