Table 3.
The sense and antisense primer sequences of the gene of interest for PCR amplification.
| Gene | Sequence | Amplified product (bp) | Annealing temp/cycles |
|---|---|---|---|
| β-actin | Sense: TCATGCCATCCTGCGTCTGGACCT Antisense: CGGACTCATCGTACTCCTGCTTG |
598 | 55 °C/35 |
| Hsp70 | Sense: GAGTCCTACGCCTTCAATATGAAG Antisense: CATCAAGAGTCTGTCTCTAGCCAA |
347 | 55 °C/35 |
| iNOS | Sense: TCTGTGCCTTTGCTCATGAC Antisense: CATGGTGAACACGTTCTTGG |
305 | 55 °C/35 |
| nNOS | Sense: CCTTCCGAAGCTTCTGGCAACAGC Antisense: TGGACTCAGATCTAAGGCGGTTGG |
474 | 66 °C/35 |
| c-fos | Sense: AGTGGTGAAGACCATGTCAGG Antisense: CATTGGGGATCTTGCAGG |
296 | 55 °C/35 |