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. 2022 Jul 15;10(7):1431. doi: 10.3390/microorganisms10071431

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of average C_T values for C. cayetanensis detection in soil samples (10 g) seeded with 100 oocysts after DNA extraction by different methods (Flotation and BAM Chapter 19b Method 1; Fast DNA^TM 50 mL SPIN kit for soil (MP Biomedicals) Method 2, Quick-DNA ^TM Fecal/Soil Microbe Midiprep kit (Zymo Research), Method 3, and DNeasy^® PowerMax^® Soil Kit (Qiagen) using bead-beating, Method 4b). Different letters on top of methods indicate statistically significant differences (p < 0.05).