Table 3.

qPCR detection data (number of positive qPCR replicates, individual C_T values) for C. cayetanensis detection in samples of soil (10 g) seeded with 20 and 10 C. cayetanensis oocysts using the flotation in dense sucrose solution method and molecular detection of the parasite.

Seeding Level	Flotation Method in Soil Samples (10 g)
	Sample Number	Number of Positive Reactions (Out of Three)	18 S CT Value (Mean ± Standard Deviation)
20 oocysts	1	2	37.9 ± 0.1
	2	3	36.9 ± 0.6
	3	3	37.7 ± 0.1
	4	2	37.9 ± 0.7
	5	1	36.5
	6	2	37.3 ± 1.9
	7	2	36.7 ± 1.3
	8	0	Und
	9	0	Und
	10	2	36.6 ± 0.0
10 oocysts	1	0	Und
	2	0	Und
	3	0	Und
	4	0	Und
	5	2	36.9 ± 0.1
	6	1	37.8
	7	2	37.6 ± 0.1
	8	0	Und
	9	0	Und
	10	0	Und

Und = Undetermined (not detected).