Table 4.
qPCR Mit1C detection data (number of positive qPCR replicates, individual CT values for C. cayetanensis and IAC for each sample) in samples of soil (10 g) seeded with 100 C. cayetanensis oocysts using different DNA isolation methods and molecular detection.
| 100 Oocysts | Flotation-Sucrose | Direct DNA Isolation-Power Max (Bead Beater) | ||||
|---|---|---|---|---|---|---|
| Sample Number | Number of Positive Reactions (Out of Three) | Mit1C CT Value (Mean ± Standard Deviation) |
IAC CT Value * (Mean ± Standard Deviation) | Number of Positive Reactions (Out of Three) | Mit1C CT Value (Mean ± Standard Deviation) | IAC CT Value * (Mean ± Standard Deviation) ** |
| 1 | 3 | 32.0 ± 0.2 | 29.5 ± 0.6 | 3 | 34.2 ± 0.6 | 27.3 ± 0.1 |
| 2 | 3 | 31.5 ± 0.1 | 29.2 ± 0.2 | 3 | 34.7 ± 0.4 | 26.8 ± 0.1 |
| 3 | 3 | 31.5 ± 0.3 | 30.0 ± 0.8 | 3 | 33.3 ± 0.6 | 29.7 ± 0.0 |
| 4 | 3 | 32.4 ± 0.3 | 28.7 ± 0.1 | 3 | 36.1 ± 0.2 | 29.3 ± 0.1 |
| 5 | 3 | 33.1 ± 1.0 | 29.6 ± 0.3 | 3 | 34.0 ± 0.8 | 29.9 ± 0.2 |
| Average | 32.1 ± 0.7 | 29.4 ± 0.5 | 34.5 ± 1.0 | 29.6 ± 0.3 | ||
| Unseeded | 0 | Und | 29.9 ± 0.1 | 0 | Und | 28.6 ± 1.4 |
* IAC of NTC in assay for Method 1 (flotation) and Method 4b (commercial direct DNA isolation from soil) = 30.3 ± 0.1. ** Out of three replicates.