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. 2022 Jul 13;11(14):2193. doi: 10.3390/cells11142193

Figure 3.

Figure 3

Hypoxia increases nuclear NF-κB p65 activity. (A) Western blot analysis of cytosolic and nuclear fractions for NF-κB p65 subunit in cardiac myocytes during normoxia or hypoxia. p65 expression is normalized to α-tubulin. * p < 0.05 versus nuclear normoxia sample. (B) Electromobility shift analysis of nuclear extracts for NF-κB p65 subunit binding derived from cardiac myocytes under normoxic and hypoxic conditions. Asterisk (*) denotes shifted protein–oligonucleotide complex, which is depleted following p65 antibody super-shift (SS). NS, non-shifted control lacking cell lysate; S, shifted sample containing p65-transfected cell lysate; CC, shift using cold competition (200x unlabeled oligonucleotides).