Figure 5.

TNFα represses PGC-1α expression and increases NF-κB p65 binding to the proximal M2 site in the PGC-1α promoter. (A) Dose-dependent repression of PGC-1α mRNA expression by TNFα, with attenuation by parthenolide, in post-natal rat cardiac myocytes. * p < 0.05 versus 0 TNFα treatment. (B) Electrophoretic mobility shift assay for p65 binding to the PGC-1α promoter proximal M2 site in response to TNFα. *, shifted protein–oligonucleotide complex. NS, non-shifted control lacking cell lysate.