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. 2022 Jul 13;11(14):2193. doi: 10.3390/cells11142193

Table 1.

Primers used for EMSA and mutagenesis (Mut.). The p65 binding site is underlined, and mutated nucleotides are in lowercase.

Primer Sequence Use
PGC1a-M2-fwd 5′-GCTTTGTCATGTGACTGGGGACTGTAGTAAGACAGGTGCCTTCAG-3′ EMSA
PGC1a-M2-rev 5′-CTGAAGGCACCTGTCTTACTACAGTCCCCAGTCACATGACAAAGC-3′ EMSA
PGC1a-∆M2-fwd 5′-GCTTTGTCATGTGACTGGaGctcGTAGTAAGACAGGTGCCTTCAG-3′ Mut.
PGC1a-∆M2-rev 5′-CTGAAGGCACCTGTCTTACTACgagCtCCAGTCACATGACAAAGC-3′ Mut.