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. 2022 Apr 5;600(10):2359–2376. doi: 10.1113/JP282999

Figure 5. Administration of 5‐aminoimidazole‐4‐carboxamide ribonucleotide (AICAR) increases activation of AMP‐activated protein kinase (AMPK) in C2C12 myotubes.

Figure 5

A, scheme of cell culture and conditioned medium (CM) transfer experiments. B, myosin heavy chain 7 (Myh7) mRNA expression levels during differentiation of C2C12 cells, n = 4 for all groups. C, Myh2 mRNA expression levels during differentiation of C2C12 cells, n = 4 for all groups. D, phosphorylated‐AMPK (p‐AMPK) (Thr172) and total AMPK protein levels in differentiated C2C12 myotubes treated with 1 mm AICAR. Protein expressions were quantified, n = 3 for all groups. E, Myh7 and Myh2 gene levels post‐AICAR treatment, n = 4 for all groups. Data are represented as means ± SD. Differences between the two groups were analysed using a two‐tailed t test. To compare more than two groups, one‐way ANOVA, Tukey's multiple comparisons test and Dunnett's test were used. P < 0.05 was considered statistically significant.