FIG. 6.

Optimizing 16S rRNA detection specificity. RNA was hybridized either in buffer that did not contain formamide (left) or in buffer containing 30% formamide (right), as described in the text. Fragmented total RNA was hybridized for 2 h at room temperature in the presence of the G. chapellei 214 chaperone-detector probe (top). Intact total RNA was hybridized overnight at room temperature (bottom).