TABLE 1.
Capture and chaperone detector probesa
| Capture probe or detector | Probe sequence (5′ to 3′) |
|---|---|
| Capture probes | |
| Geobacter chapellei-214 | AGACTCATCTGATGACAGAA |
| Geobacter arculus-214 | GGACCCATCCTGATACGGTA |
| Geobacter metallireducens-214 | GGACTCATCCAGTGACAAAA |
| Geobacter sulfurreducens-214 | GGACTCATCCGAAGACAGGA |
| Geobacter akaganeitreducens-214 | GGACTCATCCGATGTCGGGA |
| Geobacter chapellei-420 | CACAATACACTTCTTTCCCCTT |
| Geobacter arculus-420 | CCAGCCCCATTTCTTCCCTTCT |
| Geobacter metallireducens-420 | CCTCAATCACTTCTTCCCTCCC |
| Geobacter sulfurreducens-420 | TCTCAATCATTTCTTCCCTCCC |
| Desulfovibrio desulfuricans-997 | GGGAGGGTTCCGTGGATGTC |
| Desulfovibrio vulgaris-996 | GGGAGGGTCTTCCGGATGTC |
| Escherichia coli-466 | TCAATGAGCAAAGGTAT |
| Detector probes | |
| Geobacter-214 chaperone | Biotin-ACCAACTAGCTAATGGTACGC |
| Geobacter-420 chaperone | Biotin-GACAGAGCTTTACGACCCG |
| Desulfovibrio chaperone | AAACCTAGGTAAGGTTCTTC-biotin |
| Escherichia coli chaperone | ACTTTACTCCCTTCCTCC-biotin |
| Universal 519 | Biotin-CAGCAGCCGCGGTAATWC |
| Universal 915 | Biotin-GCCCCCGYCAATTYCT |
| Universal 1392 | Biotin-ACGGGCGGTGTGTRC |
a
The number after each probe name represents the approximate position of the complementary target region, utilizing G. chapellei or D. desulfuricans 16S rRNA numbering.