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FRET‐melting competition assay with a) F21T and b) F(Pu24T)T in the absence and in the presence of double‐stranded DNA competitor (ds26). Conditions: F21T or F(Pu24T)T 0.2 μM, compounds 2 μM, ds26 0, 3, or 10 μM (black, dark gray and white bars, respectively) (a) Li Caco 10 mM (pH 7.2), LiCl 90 mM and KCl 10 mM and (b) Li Caco 10 mM (pH 7.2), LiCl 99 mM and KCl 1 mM. Analyzed compounds 1–6.
