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. 2022 May 12;28(35):e202200734. doi: 10.1002/chem.202200734

Figure 6.

Figure 6

Denaturing gel electrophoresis (15 % acrylamide) of the alkylation products of a) 22AG and b) Pu24T (10 μM) in the presence of K+ buffer (10 mM Li Caco (pH 7.2), 100 mM KCl) with 1, 2, and 4 (20 μM) at increasing irradiation time (0, 0.25, 0.5, 1, 2, 4, and 8 h). Lanes 1, 8, and 15 are control experiments showing the effect in the absence of irradiation with a) 22AG and b) Pu24T. Lanes 2–7 show the results of irradiation of 22AG and Pu24T at 365 nm in the presence of 2 equiv. of 1, lanes 9–14 show the results of irradiation of 22AG and Pu24T at 365 nm in the presence of 2 equiv. of 2, and lanes 16–21 show the results of irradiation of 22AG and Pu24T at 365 nm in the presence of 2 equiv. of 4 during 0.25, 0.5, 1, 2, 4, and 8 h. On the right, quantitative analysis of the alkylation of a) the telomeric sequence 22AG and b) Pu24T oncogene (10 μM) at increasing irradiation time (0, 0.25, 0.5, 1, 2, 4, and 8 h) in the presence of 1, 2, and 4 (20 μM) or in the absence of ligand (22AG and Pu24T).