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. 2022 Apr 10;74(5):892–901. doi: 10.1002/art.42043

Figure 1.

Figure 1

Toll‐like receptor 7 (TLR‐7)–related gene expression patterns in salivary glands (SGs) from patients with IgG4‐related disease (IgG4‐RD) and TLR7‐transgenic/TLR‐7–/– mice. A, Diagram of transgenic (Tg) and knockout (KO) mice before and after stimulation with the TLR‐7 agonist R848. B, Hematoxylin and eosin (H&E)–stained and Masson's trichrome (MT)–stained sections of submandibular glands (SMGs) from representative mice. Bars = 100 μm. C and D, Fibrosis scores of SMGs (C) and serum IgG and IgG1 levels (D) in transgenic and knockout mice (n = 6 per group). E and F, Heatmaps showing differentially expressed TLR‐7–related genes in SMGs from transgenic and knockout mice (E) and from IgG4‐RD patients and healthy controls (HCs) (F). Only genes up‐regulated or down‐regulated ≥2‐fold are shown. G and H, Expression levels of mRNA for TLR‐7–related candidate genes in SGs from transgenic mice (n = 6) and knockout mice (n = 6) (G) and from healthy controls (n = 15), patients with chronic sialadenitis (CS) (n = 10), patients with Sjögren's syndrome (SS) (n = 15), and patients with IgG4‐RD (n = 15) (H). In C, D, G, and H, data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. * = P < 0.05; ** = P < 0.01, by Mann–Whitney U‐test (C, D, and G) or Kruskal–Wallis test (H). NS = not significant.