TABLE 2.
Results from probing the GenBank databasea
| Source of matching sequence | No. of identical sequences retrieved for each probe
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Am445 | Am976 | Gm633 | Gm705 | Mlb482 | Mlb662 | Mmb482 | Mmb1007 | Mlm482 | Mlm732a | Mlm732b | Mlc123 | Mlc1436 | Mcd77 | |
| Methanotrophic isolates | 36 | 26 | 16 | 33 | 8 | 11 | 13 | 13 | 14 | 7 | 4 | 5 | 6 | 3 |
| Putative methanotrophic clone from environmental samples | 33 | 5 | 8 | 24 | 22 | 9 | 1 | 3 | 7 | 3 | 0 | 0 | 0 | 0 |
| Nonmethanotrophic cultures and environmental clones | 0 | 19b | 0 | 0 | 0 | 7c | 0 | 0 | 0 | 0 | 0 | 0 | 7d | 0 |
| Unidentified isolates and environmental clones | 7 | 15 | 0 | 1 | 19 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 0 |
| Total sequences retrieved | 76 | 65 | 24 | 58 | 49 | 27 | 14 | 16 | 24 | 11 | 4 | 5 | 14 | 3 |
a
Searches were performed on 6 March 2001.
b
Of 19 nonmethanotroph sequences, 16 represent Afipia and Bosea sequences.
c
All nonmethanotroph sequences represent Cycloclasticus strains.
d
Of seven nonmethanotroph sequences, six are closely related environmental clones from putative β-Proteobacteria retrieved from an activated sludge reactor.